[ccp4bb] extra high B factor

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 29 April 2007

Previous message:
Subject: problem with HKL2000 license
From: yang li robertcatrukie {- at -} GMAIL {- dot -} COM
Date: 2007-04-29

Next message:
Subject: Re: extra high B factor
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2007-04-30

Subject: extra high B factor
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2007-04-29

Dear All:
I am refining a protein-peptide complex struture at 2.6 angstrom resolution.
The data was obtain from a co-crystal and the wilson B factor of the data is
about 70.
The affinity between protein and peptide is about 10E-7 to 10E-8 molar.
Protein fragment of the structure has a common B facor about 50.
But surprisingly, the average B factor of the peptide is as high as 130,
although the peptide can be clearly traced from the the electron density
map. All residues of the peptide have such a high B factor.
My question is how can I reduce the abnormal high B factor to a common level
or if this high B factor acceptable.
And another question is if this high B fator will influence the final
refiment level.

Thanks.

--
Jiamu Du
State Key Laboratory of Molecular Biology
Institute of Biochemistry and Cell Biology Shanghai Institutes for
Biological Sciences
Chinese Academy of Sciences (CAS)

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 29 April 2007

Previous message:
Subject: problem with HKL2000 license
From: yang li robertcatrukie {- at -} GMAIL {- dot -} COM
Date: 2007-04-29

Next message:
Subject: Re: extra high B factor
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2007-04-30