[ccp4bb] summary: tricoordinate ion?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: ccp4 6.0.99e test release
From: hari jayaram harijay {- at -} GMAIL {- dot -} COM
Date: 2008-11-04

Next message:
Subject: Summary - crystals grown from high ammonium sulphate
From: Sabine Schneider sabine {- dot -} schneider {- at -} CUP {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2008-11-05

Subject: summary: tricoordinate ion?
From: Sebastiano Pasqualato sebastiano {- dot -} pasqualato {- at -} IFOM-IEO-CAMPUS {- dot -} IT
Date: 2008-11-05

Dear all,
thanks a lot for the many replies suggesting me what to put in my
"misterious" density.
Molecules that have been suggested are: acetate, Mg ion with waters,
bicarbonate or nitrate, sulphate, borate, glycerol, tris, alternative
conformation of the Arg side chain, waters.
I have to say that, as you've noticed, the resolution is only 2.6, so
it'll be hard to conclude something beyond reasonable doubts.
What I'm doing is, as Roger Rowlett and David Borhani suggested,
calculating the maps by omitting the waters, and deciding upon the
shape of the density I see there, trying than to fit different molecules.

In doubt, it'd seem reasonable to me to place one of the molecule of
the crystallization conditions. At the moment, it seems that glycerol
could fit nicely.

I also have to apologize for the large attachment. I'm sorry I didn't
notice it was so large (I have to admit, I just sent what Wincoot
wrote out! ;-)). Next time I'll be surely posting the image to some website.

Thanks again,
all the best,
S

--
Sebastiano Pasqualato, PhD
IFOM-IEO Campus
Dipartimento di Oncologia Sperimentale
Istituto Europeo di Oncologia
via Adamello, 16
20139 - Milano
Italy

tel +39 02 9437 5094
fax +39 02 574 303 310

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: ccp4 6.0.99e test release
From: hari jayaram harijay {- at -} GMAIL {- dot -} COM
Date: 2008-11-04

Next message:
Subject: Summary - crystals grown from high ammonium sulphate
From: Sabine Schneider sabine {- dot -} schneider {- at -} CUP {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2008-11-05