[ccp4bb] sugar ligand refinement in coot

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: User proposal submission for Collaborative Crystallography at BCSB
From: Banumathi Sankaran BSankaran {- at -} LBL {- dot -} GOV
Date: 2008-11-05

Next message:
Subject: User proposal submission for Collaborative Crystallography at BCSB
From: Banumathi Sankaran BSankaran {- at -} LBL {- dot -} GOV
Date: 2008-11-05

Subject: sugar ligand refinement in coot
From: lei feng spartanfenglei {- at -} HOTMAIL {- dot -} COM
Date: 2008-11-05

hello everyone

can anyone help me with sugar ( say maltoheptaose) refinement in coot?

I use the pdb file with " GLC " name , it always breaks apart into discountinuous glucose when I try to use real space refinement , I believe that is because there is no restraint with regarding to bond , whatever in cif file.

I know I could give it a unique name , say G7 and substitute the " GLC "it in the pdb file, make a new cif file and refine it , it will not break apart again. But , when I deposit the structure in the future , it is allowed? It seems everyone working with sugar ligands are using " GLC" . how did they work in refinement ?

Thanks for any suggestion.

lei Feng
_________________________________________________________________
Color coding for safety: Windows Live Hotmail alerts you to suspicious email.
http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_safety_112008

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: User proposal submission for Collaborative Crystallography at BCSB
From: Banumathi Sankaran BSankaran {- at -} LBL {- dot -} GOV
Date: 2008-11-05

Next message:
Subject: User proposal submission for Collaborative Crystallography at BCSB
From: Banumathi Sankaran BSankaran {- at -} LBL {- dot -} GOV
Date: 2008-11-05