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Re: [ccp4bb] SUMMARY - crystallization of proteins with His-tag and/or c-myc tags
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: SUMMARY - crystallization of proteins with His-tag and/or c-myc tags
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-11-13		Next message:
Subject: Re: SUMMARY - crystallization of proteins with His-tag and/or c-myc tags
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-11-13


Subject: Re: SUMMARY - crystallization of proteins with His-tag and/or c-myc tags
From: David M Shechner shechner {- at -} MIT {- dot -} EDU
Date: 2008-11-13

> I have a more general question that's come up in discussion with former
> colleagues: what's the largest tag that has been co-crystallized with the
> target protein? I'm specifically wondering about MBP - we've encountered
> several proteins that would express decently (and, apparently, correctly
> folded) with a His-MBP tag but crashed out of solution when the tag was
> cleaved. But I don't think anyone ever tried leaving the tag on for
> crystallization trials. Or what about GST?

Hey, Nat, (et. al.),

Actually, I do know of one example using MBP. Jamie Williamson's lab used an
MBP fusion with the L30e protein in both their crystallographic and NMR
solution of its complex with RNA. Check out the following, as well as
references therein:

Chao JA, Williamson JR. (2004) Joint X-ray and NMR refinement of the yeast
L30e-mRNA complex. Structure.2004 Jul;12(7):1165-76.

Cheers,
D.S.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: SUMMARY - crystallization of proteins with His-tag and/or c-myc tags
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-11-13		Next message:
Subject: Re: SUMMARY - crystallization of proteins with His-tag and/or c-myc tags
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-11-13
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