[ccp4bb] Diffraction problems

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Program to fill unitcell randomly
From: Ethan Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-12-01

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Subject: Quikchange cloning: Insert length
From: Raji Edayathumangalam raji {- at -} BRANDEIS {- dot -} EDU
Date: 2008-12-01

Subject: Diffraction problems
From: garima singh singhg20 {- at -} GMAIL {- dot -} COM
Date: 2008-12-01

Hi All,
I am working on a 80 KDa single amino acid mutant of a protein expressed in
E.Coli. The protein is very pure and I get very nice looking crystals in
different conditions. I used different cryo protectants and tried room temp
testing of diffraction for these crystals. I don't get any diffraction at
all in most of them. A few were salt, so I was sure that there is no other
instrumental problem. If anyone has faced the same problem or someone can
give some insight about it other than the papers that suggest post
crystallization treatments , something that has worked for them, I would be
grateful

Thanks

Garima

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Program to fill unitcell randomly
From: Ethan Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-12-01

Next message:
Subject: Quikchange cloning: Insert length
From: Raji Edayathumangalam raji {- at -} BRANDEIS {- dot -} EDU
Date: 2008-12-01