[ccp4bb] Quikchange cloning: Insert length

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: garima singh singhg20 {- at -} GMAIL {- dot -} COM
Date: 2008-12-01

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Subject: O/T: can a protein which dimerizes in solution crystallize as a monomer?
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Date: 2008-12-01

Subject: Quikchange cloning: Insert length
From: Raji Edayathumangalam raji {- at -} BRANDEIS {- dot -} EDU
Date: 2008-12-01

Hi Folks,

Sorry for the non-xtallo posting.

I am curious to hear what is the longest insert anyone has cloned
using a modification of the Quikchange cloning strategy. Basically,
ligation-independent cloning by strapping on homologous regions of
the vector onto the primers which also generate the initial PCR
product. I plan to proceed with my insert which is ~ 2kb and am
curious to get some feedback if you have successfully cloned inserts
> 1.5kb using the above strategy.

Many thanks.
Raji

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Diffraction problems
From: garima singh singhg20 {- at -} GMAIL {- dot -} COM
Date: 2008-12-01