[ccp4bb] O/T: can a protein which dimerizes in solution crystallize as a monomer?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: O/T: can a protein which dimerizes in solution crystallize as a monomer?
From: "Fischmann, Thierry" thierry {- dot -} fischmann {- at -} SPCORP {- dot -} COM
Date: 2008-12-01

Dear fellow crystallographers,

This is a question which is not CCP4-related.

Is anybody aware of a protein which is known to be a dimer in solution
(say by SEC), and yet crystallizes as a monomer? Wouldn't the high
concentration in the crystallization drop further favor dimerization?

In other words, if a protein crystallizes as a monomer, can I conclude
that it does not form biologically relevant dimers in solution?

Thank you in advance for your replies.

Thierry

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Quikchange cloning: Insert length
From: Raji Edayathumangalam raji {- at -} BRANDEIS {- dot -} EDU
Date: 2008-12-01

Next message:
Subject: Re: Program to fill unitcell randomly
From: "Edward A {- dot -} Berry" BerryE {- at -} UPSTATE {- dot -} EDU
Date: 2008-12-01