[ccp4bb] LDAO & SDS-PAGE

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: CCP4 6.1.0 release and CCP4MG
From: Victor Alves vdalves {- at -} FMV {- dot -} UTL {- dot -} PT
Date: 2008-12-17

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From: Jan Abendroth Jan {- dot -} Abendroth {- at -} GMAIL {- dot -} COM
Date: 2008-12-17

Subject: LDAO & SDS-PAGE
From: Subscribe Ccp4Bb Kn L kn {- dot -} ly {- at -} AUCKLAND {- dot -} AC {- dot -} NZ
Date: 2008-12-17

Hello everyone...

I have a protein solubilised in 1% LDAO, 50 mM Phosphate pH 7.5, 150 mM
NaCl. When I mix it with laemmli buffer and heat at 95C, 3 mins for
SDS-PAGE, it aggregates (as in when you mix GuHCl with SDS) and can't be
loaded onto the gel.

Does anyone know why it happens? Thanks a lot!!!

Kien

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: CCP4 6.1.0 release and CCP4MG
From: Victor Alves vdalves {- at -} FMV {- dot -} UTL {- dot -} PT
Date: 2008-12-17

Next message:
Subject: refmac 5.5.063 vs. 5.5.066
From: Jan Abendroth Jan {- dot -} Abendroth {- at -} GMAIL {- dot -} COM
Date: 2008-12-17