Re: [ccp4bb] Transferring a Free R set.

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: ccp4-6.1.0: iMosflm libtermcap.so.2 error - SOLVED
From: Harry Powell harry {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-12-22

Next message:
Subject: Re: Refmac and MSE?
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-12-22

Subject: Re: Transferring a Free R set.
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-12-22

Alun R. Coker wrote:
> Hi All,
>
> I have been in the habit of transferring my initial free R assignments
> to any new data sets or to isomorphous data sets such as substrate
> complexes. Although theoretically this is necessary to obtain a valid
> free R many of my colleagues maintain that this is completely
> unnecessary in practice. Does anyone on the list have a view on this
> or has anyone tested to see if it makes any difference.
>
> Alun.
>
I believe it is important, especially at the initial stages of a new
refinement. Of course if you dont your FreeR values will climb remorsely
back to a proper value, but it is depressing watch it rise and means the
initial cycles are not properly weighted..
Eleanor

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: ccp4-6.1.0: iMosflm libtermcap.so.2 error - SOLVED
From: Harry Powell harry {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-12-22

Next message:
Subject: Re: Refmac and MSE?
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2008-12-22