[ccp4bb] ultrafiltration/buffer exchange device for insect cell cultures

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: problem about BALBES
From: "Dr {- dot -} F Long" fei {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-01-04

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Subject: Re: problem about BALBES
From: Xiang Liu pku06a15 {- at -} YAHOO {- dot -} COM {- dot -} CN
Date: 2009-01-04

Subject: ultrafiltration/buffer exchange device for insect cell cultures
From: Michael Hothorn hothorn {- at -} EMBL-HEIDELBERG {- dot -} DE
Date: 2009-01-04

Dear all,

sorry for the non-CCP4 question. I am expressing a protein in insect
cells that is targeted for secretion into the medium. After expression,
I want to concentrate the medium containing my protein of interest (from
10 l to 0.3 L) and at the same time change the buffer to something that
is compatible with my first ion exchange chromatography step. I would be
interested to know what kind of ultrafiltration devices can be used for
this purpose, and whether some of you could suggest a quick, reliable
and efficient system that we could purchase for our lab.

Thanks!
Michael

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: problem about BALBES
From: "Dr {- dot -} F Long" fei {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-01-04

Next message:
Subject: Re: problem about BALBES
From: Xiang Liu pku06a15 {- at -} YAHOO {- dot -} COM {- dot -} CN
Date: 2009-01-04