Re: [ccp4bb] 2D

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: ARP/wARP Solvent
From: Gerrit Langer gerrit {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-01-08

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Subject: Re: Characterization of Protein Conformational Changes
From: Roberto Mosca roberto {- dot -} mosca {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-01-08

Subject: Re: 2D
From: mesters mesters {- at -} BIOCHEM {- dot -} UNI-LUEBECK {- dot -} DE
Date: 2009-01-08

It mostly means little intermolecular contacts in one direction because
of charge repulsion, shape incomplementarity etc etc.

One thing to try is screen for additives that can help to make more
contacts between the layers or, screen for new crystal forms using
microseed matrix screening!

- J. -

Vin Purp wrote:
> Sorry for the non ccp4 related post. Can anyone point me in the direction of
> a good method, reference or link for improving 2D xtals? They are hexagonal
> 2D plates, and some have a tendency to stack. Cheers. =v=
>

--
Dr. Jeroen R. Mesters
Gruppenleiter Strukturelle Neurobiologie und Kristallogenese
Institut für Biochemie, Universität zu Lübeck
Zentrum für Medizinische Struktur- und Zellbiologie
Ratzeburger Allee 160, D-23538 Lübeck
Tel: +49-451-5004065, Fax: +49-451-5004068
Http://www.biochem.uni-luebeck.de
Http://www.iobcr.org
Http://www.selfish-brain.org
Http://www.opticryst.org
--
If you can look into the seeds of time and say
which grain will grow and which will not - speak then to me (Macbeth)
--

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: ARP/wARP Solvent
From: Gerrit Langer gerrit {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-01-08

Next message:
Subject: Re: Characterization of Protein Conformational Changes
From: Roberto Mosca roberto {- dot -} mosca {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-01-08