Re: [ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: cryoloops for X-ray data collection from protein crystals at room temperature
From: cedric bauvois cbauvois {- at -} GMAIL {- dot -} COM
Date: 2009-01-16

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Subject: Re: cryoloops for X-ray data collection from protein crystals at room temperature
From: "Schubert, Carsten [PRDUS]" CSCHUBER {- at -} ITS {- dot -} JNJ {- dot -} COM
Date: 2009-01-16

Subject: Re: cryoloops for X-ray data collection from protein crystals at room temperature
From: Guenter Fritz guenter {- dot -} fritz {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2009-01-16

Hi Cedric,
I used these, but just for testing crystals. I was afraid that the
crystal might move in the loop. For testing it worked pretty good.
http://www.jenabioscience.com/cms/en/1/catalog/733_microrttrade_room_temperature_mounting_system.html
There might be more suppliers. Please send a summary to ccp4bb.
Best
Guenter

cedric bauvois wrote:
> Dear CCP4ers,
>
> in their paper entitled " Using cryoloops for X-ray data collection
> from protein crystals at room temperature: A simple applicable method"
> ( *Journal of Crystal Growth*
>
> Volume 281, Issues 2-4
> ,
> 1 August 2005, Pages 592-595.), the authors present a way to mount
> crystals using "a cryoloop accompanied by a glass capillary cap" (see
> abstract below).
> Do you know if any commercial version of such system are now available ?
>
> Abstract: Although cryoloops are now routinely used for X-ray data
> collection from protein crystals in cryocooling condition, it is still
> necessary to collect X-ray diffraction data from protein crystals at
> room temperature under such circumstances as to find resolution limit
> and/or to avoid damage of protein crystals at cryogenic temperature
> (e.g. 100 K). Here, we show that a cryoloop, which is accompanied by a
> glass capillary cap to maintain humid environment of crystal in the
> cryoloop, can be used not only to examine protein or non-protein
> crystals but also to collect X-ray diffraction data for structural
> analysis from protein crystals at room temperature. The size of
> cryoloop should be carefully chosen so that the crystal does not move
> in the cryoloop. This crystal mounting method can be time-saving
> compared to the traditional method to mount a crystal in a glass
> capillary tube.
>
>
> Many thanks
>
> --
> Dr. Cedric Bauvois
> Cristallographie des protéines
> Institut de Recherches Microbiologiques JM Wiame -IRMW
> Av E. Gryzon 1, 1070 Brussels (Belgium)
> tél: +32 (0)2 5273634
> fax: +32 (0)2 5267273

--
***********************************

Priv.Doz.Dr. Guenter Fritz
Fachbereich Biologie
Sektion Naturwissenschaften
Universitaet Konstanz
http://www.biologie.uni-konstanz.de/fritz

Universitaetsstrasse 10
Postfach M665
D-78457 Konstanz

e-mail: Guenter.Fritz@uni-konstanz.de

Tel. Office: +49-(0)7531 88 3205
Tel. Lab : +49-(0)7531 88 3687
Fax: +49-(0)7531 88 2966

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: cryoloops for X-ray data collection from protein crystals at room temperature
From: cedric bauvois cbauvois {- at -} GMAIL {- dot -} COM
Date: 2009-01-16