Re: [ccp4bb] Summary: cryoloops for X-ray data collection from protein crystals at room temperature.

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: SUMMARY: suggestions for UV spectrometer
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-01-22

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Subject: Re: temperature after 30 minutes using microscopes ?
From: "Sandy, DJ (James)" james {- dot -} sandy {- at -} DIAMOND {- dot -} AC {- dot -} UK
Date: 2009-01-22

Subject: Re: Summary: cryoloops for X-ray data collection from protein crystals at room temperature.
From: cedric bauvois cbauvois {- at -} GMAIL {- dot -} COM
Date: 2009-01-22

hello all,

I would like to thank everyone for all suggestions.

Summary:

1 - MiTeGen Tools - MicroRT™ Room Temperature Mounting System
- crystals slipping ? (suggestion of juan: amongst other things, problem
resolved by using micromesh loops instead of using standard loops)
- uses polyester capillary which scatters xrays less than the glass
- works quite well
- everyting is reasonably airtight
- much easier to do than the old way.

links:
http://www.jenabioscience.com/cms/en/1/catalog/733_microrttrade_room_temperature_mounting_system.html
http://mitegen.com/products/micrort/micrort.shtml

2 - coat the crystal with paratone oil and mount the crystal in a standard
cryoloop
- no special tools required
- you can use the same crystal to collect under cryo-conditions and
directly compare the impact of cooling the crystal

3 - the free mounting system (FMS) from Proteros Biostructures: a humidity
control device
- it does not have highly volatile components in the conditions
- room temperature not too difficult

links:
http://www.proteros.com/articles.php?sid=18&lang=de
contact: Kris.Tesh@rigaku.com

4 - the humidifier device HC1b
- developed at the EMBL
- available at ESRF
- easy to use

links:
http://www.esrf.eu/UsersAndScience/Experiments/MX/special-setup
technical information: weatherby@embl.fr

5 - test crystal directly on plate

- japan

http://journals.iucr.org/d/issues/2002/10/01/issconts.html
http://www.labo.co.jp/contents/direct_ex.html
contact: nobuhisa@nagoya-u.jp

- France, Grenoble

explanations
http://www.natx-ray.com/products/G-Rob_2D.html
the movie
http://www.natx-ray.com/products/G-Rob_2D_movie.html
a prototype, commercialized by Natx-ray, is available on FIP beamline (
http://www.esrf.eu/exp_facilities/BM30/).*
*
contact: ferrer@ibs.fr

Best regards,
Cédric

--
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)
tél: +32 (0)2 5273634
fax: +32 (0)2 5267273

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: SUMMARY: suggestions for UV spectrometer
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-01-22

Next message:
Subject: Re: temperature after 30 minutes using microscopes ?
From: "Sandy, DJ (James)" james {- dot -} sandy {- at -} DIAMOND {- dot -} AC {- dot -} UK
Date: 2009-01-22