Re: [ccp4bb] [OFF TOPIC] his-tag doesn't bind

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Christopher Colbert Christopher {- dot -} Colbert {- at -} UTSOUTHWESTERN {- dot -} EDU
Date: 2009-01-27

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Subject: Re: [OFF TOPIC] his-tag doesn't bind
From: James Stroud xtald00d {- at -} GMAIL {- dot -} COM
Date: 2009-01-27

You don't have EDTA left over from your protease inhibitor, do you?
Some commercial cocktails include it. You may have to read the fine
print.

James

On Jan 27, 2009, at 1:00 PM, Fred wrote:

> Hi ccp4 list,
> I am trying to purify a his-tag protein by metal affinity
> chromatography. The protein was expressed in inclusion bodies and
> its his-tag doesn't bind the Qiagen Ni resin in denatured conditions
> (urea 8M or GndHCl 6M). Playing with NaCl and detergents didn't help
> much.
> Any help is appreciated.
> Fred

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: sticky crystals
From: Christopher Colbert Christopher {- dot -} Colbert {- at -} UTSOUTHWESTERN {- dot -} EDU
Date: 2009-01-27

Next message:
Subject: Re: sticky crystals
From: James Holton JMHolton {- at -} LBL {- dot -} GOV
Date: 2009-01-27