Re: [ccp4bb] Off-topic: chemical modification on thiol groups

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: small lines in diffraction pattern
From: Margriet Ovaere margriet {- dot -} ovaere {- at -} CHEM {- dot -} KULEUVEN {- dot -} BE
Date: 2009-01-28

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Subject: Re: Off-topic: chemical modification on thiol groups
From: Hannes Uchtenhagen hannes {- dot -} uchtenhagen {- at -} KI {- dot -} SE
Date: 2009-01-28

Subject: Re: Off-topic: chemical modification on thiol groups
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2009-01-28

>I am looking for a reagent (and vendor) that will irreversible put a raher
>bulky substituent on a free SH group and that does not react with free
>amines (or other potential reactive groups present on a protein surface).
>The connection with crystallography is that it is required for an
>experiment asked by a referee necessary to confirms or reject a hypothesis
>that results from a crystal structure. For this experiment it is essential
>that the reaction is not reversible (so no S-S bond formation).

Any maleimide-based fluorescent dye will be bulky, irreversible and when
done right will only go for thiols. Also easy to quantify extent of
modification.

Dima

>Remy Loris
>Vrije Universiteit Brussel

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: small lines in diffraction pattern
From: Margriet Ovaere margriet {- dot -} ovaere {- at -} CHEM {- dot -} KULEUVEN {- dot -} BE
Date: 2009-01-28

Next message:
Subject: Re: Off-topic: chemical modification on thiol groups
From: Hannes Uchtenhagen hannes {- dot -} uchtenhagen {- at -} KI {- dot -} SE
Date: 2009-01-28