Re: [ccp4bb] brute force molecular replacement

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: brute force molecular replacement
From: Clemens Vonrhein vonrhein {- at -} GLOBALPHASING {- dot -} COM
Date: 2009-02-02

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Subject: Re: brute force molecular replacement
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2009-02-02

On Feb 2, 2009, at 11:39, Clemens Vonrhein wrote:

> Hi Tassos,
>
> On Mon, Feb 02, 2009 at 11:13:28AM +0100, Anastassis Perrakis wrote:
>> To be more detailed I would:
>>
>> 0. Put the mol rep solution in ARP/wARP and let it run (in the
>> meantime
>> proceed to 1-3 if 0. fails ...)
> ^^^
>
> You didn't tell us what point 1 is:
>
>> 2. Do some density modification using the model phases (if you are
>> in ccp4
>> simplest is to use DM)
>> 3. Use the DM phases and pretend this is an 'experimental' solution
>> in
>> ARP/wARP
>
> ... are you hiding some secret, magic procedure from us?

Indeed. Point 1. was the NCS averaging for the dimeric coiled coil.
Then I realized it might not be a great idea for the coiled coil,
deleted it, and did not renumber.

Oh well - its Monday morning. And I have still not recovered from my
enthusiasm on the latest
magic of autoSHARP ;-)

A.

>
>
> Cheers
>
> Clemens
>
> --
>
> ***************************************************************
> * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com
> *
> * Global Phasing Ltd.
> * Sheraton House, Castle Park
> * Cambridge CB3 0AX, UK
> *--------------------------------------------------------------
> * BUSTER Development Group (http://www.globalphasing.com)
> ***************************************************************

P please don't print this e-mail unless you really need to
Anastassis (Tassos) Perrakis, Principal Investigator / Staff Member
Department of Biochemistry (B8)
Netherlands Cancer Institute,
Dept. B8, 1066 CX Amsterdam, The Netherlands
Tel: +31 20 512 1951 Fax: +31 20 512 1954 Mobile / SMS: +31 6 28 597791

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: brute force molecular replacement
From: Clemens Vonrhein vonrhein {- at -} GLOBALPHASING {- dot -} COM
Date: 2009-02-02