Re: [ccp4bb] Fobs - Fobs

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Barely one week remains to apply for RapiData, the BNL Course on Synchrotron Data Collection and Structure Solving.
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Subject: Re: difficult MAD dataset
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-02-02

Subject: Re: Fobs - Fobs
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-02-02

Rana Refaey wrote:
> Hi,
>
> I was wondering if anyone knows what programme I need to use to subtract the Fobs of two different crystals from each other.
>
> Regards,
> Rana
>
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Presumably the two data sets are in the same spacegroup with the same
cell dimensons more or less?

You must cad the two data sets together with suitable olumn labels and
add FC PHIC FOM from some suitable set of coordinates.

Scale them to the FC or to each other - I use program SCALEIT to do this

Then use the FFT utility to do a differenc map Fobs1 -Fobs2 PHIC FOM

Eleanor

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: difficult MAD dataset
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-02-02