[ccp4bb] putting in methionines for SeMet crystals

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: PHENIX & BSS refinement
From: Jose Casasnovas jcasasnovas {- at -} CNB {- dot -} CSIC {- dot -} ES
Date: 2009-02-03

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Subject: Re: putting in methionines for SeMet crystals
From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
Date: 2009-02-03

Subject: putting in methionines for SeMet crystals
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2009-02-03

Dear All,

I have a 9-kDa protein that crystallizes well. Since there is no structural
homologue for this molecule, I intend to make Se-Met derivative of the
protein. The molecule has no Met/Cys residues in its sequence. I wanted to
know where in the sequence should I mutate, so that the
folding/crystallizability of the protein is not compromised.

Any suggestions would be of great help.

Thanks in advance,
--
Amit Sharma, Ph.D. Research Associate, Department of Biology,
University of York, YO10 5DD UK

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: PHENIX & BSS refinement
From: Jose Casasnovas jcasasnovas {- at -} CNB {- dot -} CSIC {- dot -} ES
Date: 2009-02-03

Next message:
Subject: Re: putting in methionines for SeMet crystals
From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
Date: 2009-02-03