Re: [ccp4bb] putting in methionines for SeMet crystals

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: putting in methionines for SeMet crystals
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2009-02-03

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From: James Holton JMHolton {- at -} LBL {- dot -} GOV
Date: 2009-02-03

Subject: Re: putting in methionines for SeMet crystals
From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
Date: 2009-02-03

Hey Amit,

as your protein is tiny, maybe you can calculate a molecular replacement
model as described in Acta Cryst. (2009). D65, 169–175.

This might take longer than engineering a few methionines, though. I'd
mutate leucines.

Andreas

amit sharma wrote:
> Dear All,
>
> I have a 9-kDa protein that crystallizes well. Since there is no
> structural homologue for this molecule, I intend to make Se-Met
> derivative of the protein. The molecule has no Met/Cys residues in its
> sequence. I wanted to know where in the sequence should I mutate, so
> that the folding/crystallizability of the protein is not compromised.
>
> Any suggestions would be of great help.
>
> Thanks in advance,
> --
> Amit Sharma, Ph.D. Research Associate, Department of Biology,
> University of York, YO10 5DD UK

--
Andreas Förster, Research Associate
Paul Freemont & Xiaodong Zhang Labs
Department of Biochemistry, Imperial College London

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: putting in methionines for SeMet crystals
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2009-02-03

Next message:
Subject: Re: X-ray photon correlation length
From: James Holton JMHolton {- at -} LBL {- dot -} GOV
Date: 2009-02-03