Re: [ccp4bb] Off topic: 182 kDa protein is too large to crystallize

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Getting Molprobity to run using WinCoot
From: bernhard {- at -} CHEM {- dot -} GLA {- dot -} AC {- dot -} UK bernhard {- at -} CHEM {- dot -} GLA {- dot -} AC {- dot -} UK
Date: 2009-02-09

Next message:
Subject: Off-topic: Open Source / Freeware Binding Curve Fitting Software
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-02-09

Subject: Re: Off topic: 182 kDa protein is too large to crystallize
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2009-02-09

Hello Marek,

182 kDa protein is nothing special - unless it has huge areas of disorder,
membrane-association domains, coiled coils, or something like that. Much
larger proteins and protein complexes have been successfully crystallized.

With respect to purification - this is where you may want to diversify.
There are numerous avenues open to you: you can explore other forms of
distributive chromatography (HIC, IE, etc.); you can opt to use other
affinity matrices (dye resins, etc.) or you can try sizing using a
non-carbohydrate matrix. Not to mention that there are numerous options
for IMAC - Ni-NTA is just one of many.

I would be glad to help you further off the main list if you want to share
more details.

Cheers,

Artem

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Off-topic: Open Source / Freeware Binding Curve Fitting Software
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-02-09