Re: [ccp4bb] Se oxidation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Se oxidation
From: "Van Den Berg, Bert" Lambertus {- dot -} VandenBerg {- at -} UMASSMED {- dot -} EDU
Date: 2009-02-09

Next message:
Subject: Re: Se oxidation
From: Pascal Egea pascal {- at -} MSG {- dot -} UCSF {- dot -} EDU
Date: 2009-02-09

Subject: Re: Se oxidation
From: Michael Hothorn hothorn {- at -} EMBL-HEIDELBERG {- dot -} DE
Date: 2009-02-09

Hi,

I wouldn't bother either. I once phased a structure based on about 160
oxidized Se in the AU (Hothorn et al., JBC, 2006). Just make sure that
most of them are oxidized and do a proper absorption scan.

best
Michael

aka akaka wrote:
> Dear All
>
> I would like to know whether oxidation of Se entails any problem for
> SAD or MAD experiments and/ or how to resolve it. Cannot use DTT or
> reducing agents in my protein (extracellular and disulphide bonds are
> important).
> Thanks
>
> Dr. R.Depetris
> Weill Cornell Medical College
>
>
>
> ------------------------------------------------------------------------
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Se oxidation
From: "Van Den Berg, Bert" Lambertus {- dot -} VandenBerg {- at -} UMASSMED {- dot -} EDU
Date: 2009-02-09

Next message:
Subject: Re: Se oxidation
From: Pascal Egea pascal {- at -} MSG {- dot -} UCSF {- dot -} EDU
Date: 2009-02-09