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CCP4bb navigationCCP4bb <-- 2007 <-- May 2007 <-- 11 May 2007Subject: From: Emmanuel Prata emmanuelprata {- at -} GMAIL {- dot -} COM Date: 2007-05-11 I have a structure (with 6 dissulphide bridges) at fairly low resolution (3A) that I am trying to refine with Refmac. I've used phaser with a template with 72% sequence identity. RFZ was 3.4 and TFZ 6.6, without clashes, with 80.7% of completeness. Space group was p3121 (pointless) and Rmerge 0.12. When i tried refmac, Rfac drop to 24%, but Rfree only drop to 41%. Can anybody explain this fact? Thanks in advance, Emmanuel Prata de Souza CCP4bb navigationCCP4bb <-- 2007 <-- May 2007 <-- 11 May 2007 |
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