[ccp4bb]

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Program to evaluate RNA torsion angles?
From: Robert Immormino immormino {- at -} GMAIL {- dot -} COM
Date: 2007-05-11

Next message:
Subject: Re: . .
From: "Kerr, Iain" Iain {- dot -} Kerr {- at -} STJUDE {- dot -} ORG
Date: 2007-05-11

Subject:
From: Emmanuel Prata emmanuelprata {- at -} GMAIL {- dot -} COM
Date: 2007-05-11

Dear all,

I have a structure (with 6 dissulphide bridges) at fairly low
resolution (3A) that I am trying to refine with Refmac. I've used
phaser with a template with 72% sequence identity. RFZ was 3.4 and TFZ
6.6, without clashes, with 80.7% of completeness. Space group was
p3121 (pointless) and Rmerge 0.12. When i tried refmac, Rfac drop to
24%, but Rfree only drop to 41%.
Can anybody explain this fact?

Thanks in advance,

Emmanuel Prata de Souza

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Program to evaluate RNA torsion angles?
From: Robert Immormino immormino {- at -} GMAIL {- dot -} COM
Date: 2007-05-11

Next message:
Subject: Re: . .
From: "Kerr, Iain" Iain {- dot -} Kerr {- at -} STJUDE {- dot -} ORG
Date: 2007-05-11