Re: [ccp4bb] Eukaryotic Membrane Proteins

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: .phs file conversion
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-03-05

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Subject: Re: express large proteins in E. coli?
From: Klaus Scheffzek scheffzek {- at -} EMBL {- dot -} DE
Date: 2009-03-05

Subject: Re: Eukaryotic Membrane Proteins
From: Brad Bennett bradbennett76 {- at -} GMAIL {- dot -} COM
Date: 2009-03-05

Hi Raji-
A number of GPCRs have been overexpressed in E. coli. Examples are Reinhard
Grishammer's work with neurotensin receptor and Christopher Tate's work with
B2-adrenergic and adenosine 2a receptors. The problem was not necessarily
expression but proper outer membrane targeting of *active* receptors. This
seems to have been overcome for the most part by the use of either helpful
lipid additives like cholesterol analogs or by brute-force mutagenesis to
find more stable and active receptors. However, I don't know of any crystals
or structures from any GPCR system generated from bacteria.

Hope this helps...

Brad

On Thu, Mar 5, 2009 at 8:04 AM, Raji Edayathumangalam wrote:

> Hello Folks,
>
> My enquiry pertains to membrane proteins. If you have been able to
> successfully express a eukaryotic integral MEMBRANE protein in E. coli or
> know of such a case, would you please provide some details.
>
> Thanks.
> Raji
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: .phs file conversion
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-03-05

Next message:
Subject: Re: express large proteins in E. coli?
From: Klaus Scheffzek scheffzek {- at -} EMBL {- dot -} DE
Date: 2009-03-05