Re: [ccp4bb] express large proteins in E. coli?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Eukaryotic Membrane Proteins
From: Brad Bennett bradbennett76 {- at -} GMAIL {- dot -} COM
Date: 2009-03-05

Next message:
Subject: Re: Problem with setting up ccp4 gui
From: Ed Pozharski epozh001 {- at -} UMARYLAND {- dot -} EDU
Date: 2009-03-05

Subject: Re: express large proteins in E. coli?
From: Klaus Scheffzek scheffzek {- at -} EMBL {- dot -} DE
Date: 2009-03-05

David Garboczi wrote:
> Have large proteins (300+ kD) been successfully produced in E. coli?
> Which ones?

have a look at Suo et al., 2000, PNAS, 14188-14193; (350 kDa);
or Pan et al., 1999, NAR, 1094-1103; ('only' 190 kDa).

klaus

>
> Dave
>

--
************************************************************************

Dr. Klaus Scheffzek
European Molecular Biology Laboratory (EMBL)
Structural and Computational Biology Unit
Meyerhofstr. 1
69117 Heidelberg, Germany

Phone: (49) 6221 387 401
Fax: (49) 6221 387 306/519
E-mail: scheffzek@embl.de
Web: www.embl.de

************************************************************************

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Eukaryotic Membrane Proteins
From: Brad Bennett bradbennett76 {- at -} GMAIL {- dot -} COM
Date: 2009-03-05

Next message:
Subject: Re: Problem with setting up ccp4 gui
From: Ed Pozharski epozh001 {- at -} UMARYLAND {- dot -} EDU
Date: 2009-03-05