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Re: [ccp4bb] Superdex 200 PC columns
 

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From: Roger Rowlett RRowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2007-05-12 		Next message:
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From: "Herbert J {- dot -} Bernstein" yaya {- at -} BERNSTEIN-PLUS-SONS {- dot -} COM
Date: 2007-05-13


Subject: Re: Superdex 200 PC columns
From: Andrew Beavil andrew {- dot -} beavil {- at -} KCL {- dot -} AC {- dot -} UK
Date: 2007-05-13
You might also like to consider the new Superdex 200 'short column' 5x150mm.
I have just ordered one as it promises a rapid view of aggregation state
with a max flow rate of up to 0.8ml/min. The column volume is 3ml ie
3.75mins per run.

There are some pdf brochures on the GE web site which show reasonable
separation compared to the standard 10x300mm (24ml) columns.

Has anyone tried one yet ? - released in feb 2007

best wishes,

Andrew

----- Original Message -----
From: "Robert Cleverley"
To:
Sent: Saturday, May 12, 2007 11:30 AM
Subject: [ccp4bb] Superdex 200 PC columns


> Sorry for perhaps off-topic question but I am writing to ask if anyone has
> experience in using Amersham Superdex 200 PC 3.2/30 columns in conjuction
> with
> the Precision Column holder on a standard (not SMART system) AKTA FPLC.
> At the
> moment using a Superdex 200 10/300 column on a standard AKTA I can get a
> reasonable signal from down to about 20 ug protein - I am wondering how
> much
> less protein I will be able to use with the narrower Superdex 200 PC
> 3.2/30
> column when I hook it up to the same FPLC via the Precision Column holder.
>




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