Re: [ccp4bb] images

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Question about stereo on LCD monitor
From: Warren DeLano warren {- at -} DELSCI {- dot -} COM
Date: 2009-03-20

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Subject: Re: Problems with phasing a protein (1300aa)
From: Ho-Leung Ng holeung {- at -} BERKELEY {- dot -} EDU
Date: 2009-03-20

Subject: Re: images
From: Scott Classen sclassen {- at -} LBL {- dot -} GOV
Date: 2009-03-20

On Mar 19, 2009, at 3:26 AM, Andrew Purkiss-Trew wrote:

> On Wed, 2009-03-18 at 18:19 +0000, Frank von Delft wrote:
>> Maybe, but images without experimental context (sequence? ligands?
>> purification? crystallization format? -- PURPOSE OF EXPERIMENT!?!!
>> relationship to the other 15 similar datasets) are as good as no
>> images. And as far as I know, there's no good discussion on the
>> table
>> for that. At least, no-one on the thread mentioned it, so they're
>> probably not thinking about it either.
>>
>> I suppose efforts like PIMS or are a start, and maybe they can even
>> have
>> enough information (my feeling is they currently don't). But that's
>> where the discussion should start: how to index (in sense of
>> annotate)
>> the datasets. The technicalities are just that: technicalities.
>>
>> Or even closer to home: does ANY detector/beamline write even
>> timestamps
>> into the image header...? Never mind ring current, intensity of the
>> beam, size of beam, size of crystal, length of direct beam path, etc
>> etc...
>>
>
> As far as I know, most detectors write the current time into the image
> header. Certainly our in house MAR image plate systems do, as do the
> detectors at Diamond and ESRF (for those that I've looked at this
> morning).

FYI at my beamline (ALS 12.3.1), in addition to the usual useful
metadata, we also put in the beamline ID and the serial number of the
detector. In theory anything can be added if you take the time to
customize the detector code.

HEADER_BYTES= 512;
DIM=2;
BYTE_ORDER=little_endian;
TYPE=unsigned_short;
SIZE1=3072;
SIZE2=3072;
PIXEL_SIZE=0.102592;
BIN=2x2;
BIN_TYPE=HW;
ADC=fast;
CREV=1;
BEAMLINE=ALS1231;
DETECTOR_SN=907;
DATE=Tue Feb 3 11:07:38 2009;
TIME=10.000000;
ACC_TIME=11516;
DISTANCE=649.80;
TWOTHETA=0.00;
PHI=191.310;
OSC_START=191.310;
OSC_RANGE=1.000;
WAVELENGTH=1.033184;
BEAM_CENTER_X=155.70;
BEAM_CENTER_Y=157.40;
DENZO_X_BEAM=157.76;
DENZO_Y_BEAM=155.70;

Scott

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Question about stereo on LCD monitor
From: Warren DeLano warren {- at -} DELSCI {- dot -} COM
Date: 2009-03-20

Next message:
Subject: Re: Problems with phasing a protein (1300aa)
From: Ho-Leung Ng holeung {- at -} BERKELEY {- dot -} EDU
Date: 2009-03-20