[ccp4bb]

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Agenda for BNL imgCIF workshop
From: "Herbert J {- dot -} Bernstein" yaya {- at -} BERNSTEIN-PLUS-SONS {- dot -} COM
Date: 2007-05-13

Next message:
Subject: Re: Low resolution model building
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-05-14

Subject:
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-05-14

I presume you tried P32 21 also? Pointless cannot distinguish the
difference..

Refinement at 3A is tricky - you can overfit quite easily and maybe
Rfree is correctly flagging that there are still errors.
Can you see features which need rebuilding?
Eleanor

Emmanuel Prata wrote:
> Dear all,
>
> I have a structure (with 6 dissulphide bridges) at fairly low
> resolution (3A) that I am trying to refine with Refmac. I've used
> phaser with a template with 72% sequence identity. RFZ was 3.4 and TFZ
> 6.6, without clashes, with 80.7% of completeness. Space group was
> p3121 (pointless) and Rmerge 0.12. When i tried refmac, Rfac drop to
> 24%, but Rfree only drop to 41%.
> Can anybody explain this fact?
>
> Thanks in advance,
>
> Emmanuel Prata de Souza
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Agenda for BNL imgCIF workshop
From: "Herbert J {- dot -} Bernstein" yaya {- at -} BERNSTEIN-PLUS-SONS {- dot -} COM
Date: 2007-05-13

Next message:
Subject: Re: Low resolution model building
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-05-14