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[ccp4bb] OT: Crystallisation compatible detergents
- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: two identical proteins in one asymmetric unit
From: Folmer Fredslund folmerf {- at -} GMAIL {- dot -} COM
Date: 2009-03-24		Next message:
Subject: Re: two identical proteins in one asymmetric unit
From: Jim Fairman fairman {- dot -} jim {- at -} GMAIL {- dot -} COM
Date: 2009-03-24


Subject: OT: Crystallisation compatible detergents
From: Darren Hart hart {- at -} EMBL {- dot -} FR
Date: 2009-03-24

Hello,
We can make a nice 1:1 complex between two proteins that then gradually
precipitates during storage at 4 degC. With 0.005% Tween-20 the complex is
stable and does not preciptate noticeably. We have used this in buffers to
measure the kinetics of binding by Biacore so it is clearly compatible with
"functionality".

Is Tween-20 at this concentration compatible with crystallisation? Is it
worth giving a go, or a waste of time? Should we try and remove it, or
reprepare the complex in an alternative detergent?

If we need to try an alternative, what would people recommend?

Thanks in advance for your suggestions,
Darren

--
**********************************************************************
Dr. Darren Hart,
Team Leader
High Throughput Protein Lab
Grenoble Outstation
European Molecular Biology Laboratory (EMBL)
**********************************************************************
EMBL webpages:
http://tinyurl.com/6xdltl
http://tinyurl.com/667jrp

Email: hart@embl.fr
Tel: +33 4 76 20 77 68
Fax: +33 4 76 20 71 99
Skype: hartdarren
Postal address: EMBL, 6 rue Jules Horowitz, BP181, 38042 Grenoble, Cedex
9, France
**********************************************************************

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: two identical proteins in one asymmetric unit
From: Folmer Fredslund folmerf {- at -} GMAIL {- dot -} COM
Date: 2009-03-24		Next message:
Subject: Re: two identical proteins in one asymmetric unit
From: Jim Fairman fairman {- dot -} jim {- at -} GMAIL {- dot -} COM
Date: 2009-03-24
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