Re: [ccp4bb] Crystal vacuum cleaner

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: selenomethionine labeling
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2009-03-26

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Subject: Re: Crystal vacuum cleaner
From: Watanabe Nobuhisa nobuhisa {- at -} NUSRC {- dot -} NAGOYA-U {- dot -} AC {- dot -} JP
Date: 2009-03-26

Subject: Re: Crystal vacuum cleaner
From: Jim Pflugrath Jim {- dot -} Pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2009-03-26

If I understand you correctly, you are writing about sucking a crystal up
into a small capillary. This is an ancient technique used with the very
first protein crystal diffraction data collection in the 1950s and 1960s
that is still used today. You can use a small 1 ml plastic syringe for the
positive/negative pressure if you connect it to the capillary by a small
piece of rubber tubing.

There has even been a least one paper published on freezing crystals in such
capillaries.

Jim

_____

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob
Keller
Sent: Thursday, March 26, 2009 2:43 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystal vacuum cleaner

Dear Crystallographers,

Has anybody ever heard of mounting crystals in tiny crystal-sized
capillaries, such as are pulled by patch-pipet machines, or those used in
microfluidics? The material could be either glass or plastic, and one could
have some method of continuous positive or negative pressure, perhaps
through a hole in the crystal cap. Anyway, once safely inside the tiny
capilary, one could freeze it at leisure, without concern for evaporation.
It would really make harvesting easy--just vacuum up the crystal, then plop
in LiqN2/propane as per usual. I guess it could also really be done with
appropriate modification of a micro-manipulator.

Jacob

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-keller2@northwestern.edu
*******************************************

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: selenomethionine labeling
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2009-03-26

Next message:
Subject: Re: Crystal vacuum cleaner
From: Watanabe Nobuhisa nobuhisa {- at -} NUSRC {- dot -} NAGOYA-U {- dot -} AC {- dot -} JP
Date: 2009-03-26