[ccp4bb] IPTG induced protein expression

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Halide soaking
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-03-31

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Subject: Error with molrep when using locked srf.
From: Francis E Reyes Francis {- dot -} Reyes {- at -} COLORADO {- dot -} EDU
Date: 2009-03-31

Subject: IPTG induced protein expression
From: nagem {- at -} ICB {- dot -} UFMG {- dot -} BR nagem {- at -} ICB {- dot -} UFMG {- dot -} BR
Date: 2009-03-31

Dear CCP4bb users,

I was wondering if anyone has used regular IPTG (not IPTG-dioxane free, special
grade) for protein expression.
Are there any problems using such regular samples (5mg dioxane per Kg of IPTG)
for protein expression?

It would be great to have an idea for real problems if using this.

Cheers

Ronaldo.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Halide soaking
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-03-31

Next message:
Subject: Error with molrep when using locked srf.
From: Francis E Reyes Francis {- dot -} Reyes {- at -} COLORADO {- dot -} EDU
Date: 2009-03-31