Re: [ccp4bb] Building structure in COOT

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Error with molrep when using locked srf.
From: Alexei Vagin alexei {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-04-01

Next message:
Subject: [ANNOUNCE] Journal of Failed Crystallization Experiments
From: Sehl Oediter sehloediter {- at -} GMAIL {- dot -} COM
Date: 2009-04-01

Subject: Re: Building structure in COOT
From: Paul Emsley paul {- dot -} emsley {- at -} BIOCH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-04-01

David Cobessi wrote:
> Liew Chong Wai wrote:
>> Hi,
>>
>> Good day
>> I am currently building my structure by using COOT. My protein is a
>> tetramer protein and I have fit my protein sequence into one of the
>> monomer of the homologous model. May I know how can I replace other
>> monomer with the amended monomer??
>> Thanks
>>
>> *Chong-wai*
>>
> Hi Chong-wai,
> Several possibilities to generate the tetramer from the monomer, let
> say A.
> You can calculate the RT matrices ( in CCP4 or using LSQMAN and
> MOLEMAN2 or using O for example) to superpose the monomer A onto B, C
> and D and then apply these matrices to the amended monomer (A) in
> order to generate the tetramer.
> You can also superpose the monomer A onto B in COOT by using SSM or
> LSQ and save the new position as monomer B in COOT.... etc... and then
> you can generate 4 monomers and the tetramer. Do not forget to change
> the chain ID before refinement.
>

Blimey.

Extensions -> NCS -> Copy NCS Chain...

See Section 5.27 of the user manual for more information.

http://www.ysbl.york.ac.uk/~emsley/coot/doc/chapters/user-manual_5.html#SEC137

Paul

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Error with molrep when using locked srf.
From: Alexei Vagin alexei {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2009-04-01

Next message:
Subject: [ANNOUNCE] Journal of Failed Crystallization Experiments
From: Sehl Oediter sehloediter {- at -} GMAIL {- dot -} COM
Date: 2009-04-01