Re: [ccp4bb] Building structure in COOT

- Protein crystallography

Main steps:

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- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Choosing weighing term
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2009-04-01

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Date: 2009-04-01

Subject: Re: Building structure in COOT
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2009-04-01

On Apr 1, 2009, at 4:35, Liew Chong Wai wrote:

> Hi,
>
> Good day
> I am currently building my structure by using COOT. My protein is a
> tetramer protein and I have fit my protein sequence into one of the
> monomer of the homologous model. May I know how can I replace other
> monomer with the amended monomer??
> Thanks
>

I admit that a trick I found useful, is after building one chain do a
molecular replacement with this as a model and lookign for four
copies. Then go back to Coot ... avoids thinking about rotations
matrixes which is always a challenge (for me that is).

Tassos

> Chong-wai
>

P please don't print this e-mail unless you really need to
Anastassis (Tassos) Perrakis, Principal Investigator / Staff Member
Department of Biochemistry (B8)
Netherlands Cancer Institute,
Dept. B8, 1066 CX Amsterdam, The Netherlands
Tel: +31 20 512 1951 Fax: +31 20 512 1954 Mobile / SMS: +31 6 28 597791

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Choosing weighing term
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2009-04-01