[ccp4bb]

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject:
From: KUMARASWAMI MUTHIAH megunagu {- at -} HOTMAIL {- dot -} COM
Date: 2009-04-01

Next message:
Subject: Re: protein-progesterone or estrogen complexes
From: Kendall Nettles knettles {- at -} SCRIPPS {- dot -} EDU
Date: 2009-04-01

Subject:
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2009-04-01

Unless you want a ligand free molecule it might be a good idea to add the substance already to the growing Ecoli (?) culture. That has proven to be successful for such molecules.

Best wishes

Kornelius

On Wed, 1 Apr 2009 16:59:05 +0000
KUMARASWAMI MUTHIAH wrote:
>
> Anybody tried to cocrystallize the protein-progesterone or estrogen complexes, if so how do you go about the solubility of these compounds? Progesterone is only soluble in 50% chloroform or 100% DMSO and the dilution of this stock is not possible as chloroform falls out of solution. Lots of papers out there used soaking with progesterone or expressed the protein in the presence of progesterone. Any suggestions would be appreciated.Thanks
>
>
>
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----------------------------------------------
Kornelius Zeth
Max Planck Institute for Developmental Biology
Dept. Protein Evolution
Spemannstr. 35
72076 Tuebingen, Germany
kornelius.zeth@tuebingen.mpg.de
Tel -49 7071 601 323
Fax -49 7071 601 349

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject:
From: KUMARASWAMI MUTHIAH megunagu {- at -} HOTMAIL {- dot -} COM
Date: 2009-04-01

Next message:
Subject: Re: protein-progesterone or estrogen complexes
From: Kendall Nettles knettles {- at -} SCRIPPS {- dot -} EDU
Date: 2009-04-01