Re: [ccp4bb] Acrylamide in RNA crystallization

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Acrylamide in RNA crystallization
From: vanessa delfosse vanessa {- dot -} delfosse {- at -} GMAIL {- dot -} COM
Date: 2009-04-01

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Subject: Re: Acrylamide in RNA crystallization
From: Francis E Reyes Francis {- dot -} Reyes {- at -} COLORADO {- dot -} EDU
Date: 2009-04-01

Are you talking about chunks of acrylamide? Or perhaps trace molecules?

As for chunks, we normally pass it through a 0.2 um filter before
setting up trays.

FR

On Apr 1, 2009, at 4:48 PM, vanessa delfosse wrote:

> Dear all,
>
> I'm currently trying to crystallize a 70 nt RNA and I would like to
> know if the presence of acrylamide, some traces from the
> purification step, may interfere with crystallization of nucleic
> acids ?
>
> Thanks in advance,
>
> Vanessa.
>
>
> --
> ============================
> Vanessa Delfosse - PhD
>
> Universite de Montreal
> Departement de Biochimie
> CP 6128 Succ. Centre Ville
> Montreal QC H3C 3J7
> CANADA
>
> tel : 514 343-6111 3780
> @ : v.delfosse@umontreal.ca
> ============================

---------------------------------------------
Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Acrylamide in RNA crystallization
From: vanessa delfosse vanessa {- dot -} delfosse {- at -} GMAIL {- dot -} COM
Date: 2009-04-01

Next message:
Subject: Glycerol for crystal screens
From: Matthew Alan Bratkowski mab259 {- at -} CORNELL {- dot -} EDU
Date: 2009-04-01