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[ccp4bb]
- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

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   - X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Vacancies at Diamond Light Source
From: "Hannon, TJ (Tim)" tim {- dot -} hannon {- at -} DIAMOND {- dot -} AC {- dot -} UK
Date: 2009-04-02		Next message:
Subject: CCP4 tcl installation problem (mac)
From: Simon Kolstoe s {- dot -} kolstoe {- at -} UCL {- dot -} AC {- dot -} UK
Date: 2009-04-02


Subject:
From: Marc Graille marc {- dot -} graille {- at -} U-PSUD {- dot -} FR
Date: 2009-04-02

Hi,
We had success using a testosterone hemisuccinate derivative which is
much more soluble.
For details, see: Drevelle et al; J Mol Biol. 'jour', 'J Mol Biol.');> 2006 Apr 28;358(2):455-71

Hope this helps!!
Marc

KUMARASWAMI MUTHIAH a écrit :
> Anybody tried to cocrystallize the protein-progesterone or estrogen
> complexes, if so how do you go about the solubility of these
> compounds? Progesterone is only soluble in 50% chloroform or 100% DMSO
> and the dilution of this stock is not possible as chloroform falls out
> of solution. Lots of papers out there used soaking with progesterone
> or expressed the protein in the presence of progesterone. Any
> suggestions would be appreciated.
> Thanks
>
>
>
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--
Marc Graille, PhD
Yeast Structural Genomics, IBBMC CNRS UMR8619 Bat 430 Universite Paris Sud 91405 Orsay Cedex
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Tel: (33) 1 69 15 31 57
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