[ccp4bb] Link two proteins into one polypeptide

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: cryoprotectant for 1,6 hexanediol
From: Jürgen_Bosch jubosch {- at -} JHSPH {- dot -} EDU
Date: 2009-04-02

Next message:
Subject: Postdoctoral position available
From: Stewart Turley turley {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2009-04-02

Subject: Link two proteins into one polypeptide
From: Raji Edayathumangalam raji {- at -} BRANDEIS {- dot -} EDU
Date: 2009-04-02

Hi People,

Could anyone point me to successful examples for two unrelated
proteins that have been stitched together into one single polypeptide
chain with flexible amino acids to create a functional chimera that
was subsequently crystallized. I've looked up a few.

I am particularly interested in understanding all the important
considerations while designing a flexible linker even though many of
these factors might be case dependent might be variable, obvious and
commonsensical. Either way, I'd like to hear what folks have to say.

Thanks very much.
Raji

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: cryoprotectant for 1,6 hexanediol
From: Jürgen_Bosch jubosch {- at -} JHSPH {- dot -} EDU
Date: 2009-04-02

Next message:
Subject: Postdoctoral position available
From: Stewart Turley turley {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2009-04-02