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Re: [ccp4bb] Cross-crystal density averaging and/or refinement

- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

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   - X-ray detectors

Basic tutorials:

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Cross-crystal density averaging and/or refinement
From: Ethan Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2009-04-06
Next message:
Subject: Re: Manganese buffers for protein crystallization
From: Charlie Bond Charles {- dot -} Bond {- at -} UWA {- dot -} EDU {- dot -} AU
Date: 2009-04-06


Subject: Re: Cross-crystal density averaging and/or refinement
From: Jürgen_Bosch jubosch {- at -} JHSPH {- dot -} EDU
Date: 2009-04-06

Hi Ethan,

give Eden a chance. It's a pain to get it running but once you've
overcome memory addressing issues it runs like a dream and
significantly improves your remaining electron density map. Or if you
are not that desperate yet you could run GraphEnt, helped me quite a
bit - here's the link: http://utopia.duth.gr/~glykos/graphent.html

Jürgen


-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Biochemistry and Molecular Biology, W8708
615 North Wolfe Street
Baltimore, MD 21205
Phone: +1-410-614-4742
Fax: +1-410-955-3655
http://web.me.com/bosch_lab/

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Cross-crystal density averaging and/or refinement
From: Ethan Merritt merritt {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2009-04-06
Next message:
Subject: Re: Manganese buffers for protein crystallization
From: Charlie Bond Charles {- dot -} Bond {- at -} UWA {- dot -} EDU {- dot -} AU
Date: 2009-04-06



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