Re: [ccp4bb] MolRep with Phaser using a partial existing solution

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Refmac5 restrained refinement could not keep the changes made in Turbo
From: Tim Grune tim {- dot -} grune {- at -} SYNCHROTRON {- dot -} VIC {- dot -} GOV {- dot -} AU
Date: 2007-05-17

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Subject: Re: MolRep with Phaser using a partial existing solution
From: Tim Grune tim {- dot -} grune {- at -} SYNCHROTRON {- dot -} VIC {- dot -} GOV {- dot -} AU
Date: 2007-05-17

Subject: Re: MolRep with Phaser using a partial existing solution
From: Edward Berry eaberry {- at -} LBL {- dot -} GOV
Date: 2007-05-17

What about giving the correctly positioned partial solution,
and fixed angles/translation of 0,0,0,0,0,0 for that part?

Jay Thompson wrote:

> Hi,
>
> I have a question with molecular replacement using Phaser. I'm trying
> to solve a complex and I have a partial molecular replacement solution
> solved using another program. This solution is correct and makes up
> ~50% of the entire complex. I wanted to fix this solution and search
> for another small fragment of the complex using Phaser. I've been
> reading the Phaser manual and it seems that I cannot input a pdb with
> this partial solution and tell the program to fix this molecule. It
> seems that fixed solutions can be only input by putting in its Euler
> angles and fractional coordinate translations. Is this correct that I
> cannot input a pdb and fix it? If I cannot do this, then is there a
> quick way to identify the euler angles and coordinate translations for
> Phaser.
>
> Thanks a lot!!
>
> Jay

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