Re: [ccp4bb] MolRep with Phaser using a partial existing solution

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: MolRep with Phaser using a partial existing solution
From: Edward Berry eaberry {- at -} LBL {- dot -} GOV
Date: 2007-05-17

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Subject: Re: MolRep with Phaser using a partial existing solution
From: Jay Thompson jay {- dot -} xtalman {- at -} GMAIL {- dot -} COM
Date: 2007-05-17

Subject: Re: MolRep with Phaser using a partial existing solution
From: Tim Grune tim {- dot -} grune {- at -} SYNCHROTRON {- dot -} VIC {- dot -} GOV {- dot -} AU
Date: 2007-05-17

Hello,

you could try to let phaser search for the first fragment, too. That way it
should produce a file ending with '.sol' which you can pass to phaser for the
second search (bottom at the ccp4-gui, "Define search sets...").

Otherwise, if you do not want to move the first fragment, both the Euler
angles and the translation should be 0 0 0.

Tim

On Friday 18 May 2007 11:24, Jay Thompson wrote:
> Hi,
>
> I have a question with molecular replacement using Phaser. I'm trying to
> solve a complex and I have a partial molecular replacement solution solved
> using another program. This solution is correct and makes up ~50% of the
> entire complex. I wanted to fix this solution and search for another small
> fragment of the complex using Phaser. I've been reading the Phaser manual
> and it seems that I cannot input a pdb with this partial solution and tell
> the program to fix this molecule. It seems that fixed solutions can be
> only input by putting in its Euler angles and fractional coordinate
> translations. Is this correct that I cannot input a pdb and fix it? If I
> cannot do this, then is there a quick way to identify the euler angles and
> coordinate translations for Phaser.
>
> Thanks a lot!!
>
> Jay

--
Tim Grune
Australian Synchrotron
800 Blackburn Road
Clayton, VIC 3168
Australia

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: MolRep with Phaser using a partial existing solution
From: Edward Berry eaberry {- at -} LBL {- dot -} GOV
Date: 2007-05-17

Next message:
Subject: Re: MolRep with Phaser using a partial existing solution
From: Jay Thompson jay {- dot -} xtalman {- at -} GMAIL {- dot -} COM
Date: 2007-05-17