Re: [ccp4bb] MolRep with Phaser using a partial existing solution

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From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-05-18

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From: STEWART TURLEY turley {- at -} U {- dot -} WASHINGTON {- dot -} EDU
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Subject: Re: MolRep with Phaser using a partial existing solution
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-05-18

Jay Thompson wrote:
> Hi,
>
> I have a question with molecular replacement using Phaser. I'm trying
> to solve a complex and I have a partial molecular replacement solution
> solved using another program. This solution is correct and makes up
> ~50% of the entire complex. I wanted to fix this solution and search
> for another small fragment of the complex using Phaser. I've been
> reading the Phaser manual and it seems that I cannot input a pdb with
> this partial solution and tell the program to fix this molecule. It
> seems that fixed solutions can be only input by putting in its Euler
> angles and fractional coordinate translations. Is this correct that I
> cannot input a pdb and fix it? If I cannot do this, then is there a
> quick way to identify the euler angles and coordinate translations for
> Phaser.
>
> Thanks a lot!!
>
> Jay
Euler angles 0 0 0 translation 0 0 0
That leaves it exactly where it is..
Eleanor

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