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[ccp4bb] Research Scientist position available |
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CCP4bb navigationCCP4bb <-- 2007 <-- May 2007 <-- 18 May 2007Subject: Research Scientist position available From: STEWART TURLEY turley {- at -} U {- dot -} WASHINGTON {- dot -} EDU Date: 2007-05-18 Thanks Stewart RESEARCH SCIENTIST POSITION AVAILABLE Molecular Biology for Structural Studies on Proteins from Global Pathogens JOB DESCRIPTION: The projects in the protein crystallography group of Wim Hol are all focused on providing a basis for development of new therapeutics to combat tropical diseases. Current projects include molecular machines, protein-RNA complexes and integral membrane proteins. In particular the sophisticated Type 2 Protein Secretion system of cholera toxin, the unique RNA editing machinery in mitochondria of the sleeping sickness parasite, and the essential malaria parasite invasion machinery are at the center of attention. The responsibilities of the successful candidate include: (i) Design and construct plasmids and multi-plasmid systems to express and co-express protein of interest in E. coli, and in other expression systems like insect cells; (ii) Carry out site directed mutagenesis to obtain variants of the proteins under investigation with respect to length, active site characteristics, surface properties, etc.; (iii) Small scale expression of proteins and multi-protein complexes; (iv) Prepare large quantities of RNA by in vitro translation methods; (v) Maintain molecular biology, protein expression and protein purification equipment; (vi) Maintain the plasmid and expression system database of the group; Further information regarding the work of the laboratory can be found at: www.bmsc.washington.edu/WimHol depts.washington.edu/biowww/faculty/hol.html JOB REQUIREMENTS: - At least three years of experience with: - Constructing vectors with optimal properties for producing protein with various cleavable and non-cleavable affinity-chromatography purification tags. - Site-directed mutagenesis; - Over-expression of eukaryotic proteins in E. coli or other heterologous expression systems; - Management of databases containing plasmid, vector and expression information. - Maintaining molecular biology equipment. - Molecular biology software and web-based programs. - Ability to work on multiple projects simultaneously with minimal supervision. - Excellent interpersonal skills to function optimally in the project teams and to cooperate with collaborators in other institutions. - B. Sc. in biochemistry or microbiology, or equivalent. DESIRED SKILLS (not required): - Experience with production of large quantities of RNA by in vitro translation methods; and/or protein expression using baculo virus and insect cells; and/or application of phage display techniques. Interested candidates are invited to send an application plus brief CV plus names and email addresses of three references to: Wim Hol at wghol@u.washington.edu CCP4bb navigationCCP4bb <-- 2007 <-- May 2007 <-- 18 May 2007 |
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