[ccp4bb] Problems in MR

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: SUMMARY: Main topic of the day: Protein crystallization
From: Ronaldo Alves Pinto Nagem nagem {- at -} ICB {- dot -} UFMG {- dot -} BR
Date: 2007-03-05

Next message:
Subject: SUMMARY: Cannot run NTA to purify the protein having Histag?
From: Ngo Duc Tri ngoductri {- at -} GMAIL {- dot -} COM
Date: 2007-03-05

Subject: Problems in MR
From: thunderbird thunderbird {- at -} MOON {- dot -} IBP {- dot -} AC {- dot -} CN
Date: 2007-03-05

Hi all.
I am trying to solve a structure with one molecules in the asu through MR.
Using a not good data set at 3.0A and a structure with sequence similarity of
37%, Phaser gave a result of z score 14.7 and LL-gain 220, probably a right
solution.
But when I slightly modified the model I got from Phaser and used it as an
input to run Phaser again, I got another solution overlapped partly with the
first solution. I don't know how it could happen like that. And the Rfree
factor could not go
below 0.43 in the following refinemet. I am wondering what is wrong with it?
thanx in advance
Lei Yin

Lei Yin, Ph.D.
National Laboratory of Biomacromolecules
Institute of Biophysics
Chinese Academy of Sciences
No. 15 Datun Road, Beijing 100101, China
E-mail:thunderbird@moon.ibp.ac.cn
Tel: 86-10-64888548

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: SUMMARY: Main topic of the day: Protein crystallization
From: Ronaldo Alves Pinto Nagem nagem {- at -} ICB {- dot -} UFMG {- dot -} BR
Date: 2007-03-05

Next message:
Subject: SUMMARY: Cannot run NTA to purify the protein having Histag?
From: Ngo Duc Tri ngoductri {- at -} GMAIL {- dot -} COM
Date: 2007-03-05