[ccp4bb] ´ð¸´: [ccp4bb] Cryo-protectant

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Cryo-protectant
From: Liew Chong Wai CWLiew {- at -} NTU {- dot -} EDU {- dot -} SG
Date: 2009-04-23

Next message:
Subject: Re: Cryo-protectant
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-04-24

Subject: ´ð¸´: Cryo-protectant
From: Li Sheng biolish {- at -} GMAIL {- dot -} COM
Date: 2009-04-23

Hi Liew,

Have you ever tried paratone or some other oil?

Sincerely,

Li

__________________________________________
Email:sli@sibs.ac.cn
Institute of Biochemistry and Cell Biology
Institutes for Biological Sciences
Chinese Academy of Sciences
320 Yue-Yang Road, Shanghai 200031, China
Tel: +86-21-5492-1217
__________________________________________

·¢¼þÈË: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] ´ú±í Liew Chong
Wai
·¢ËÍÊ±¼ä: 2009Äê4ÔÂ24ÈÕ 11:47
ÊÕ¼þÈË: CCP4BB@JISCMAIL.AC.UK
Ö÷Ìâ: [ccp4bb] Cryo-protectant

Hi all,

Good day

I used MPD as a cryoprotectant (20%, 30%) for my crystal. However, there is
no diffraction signal at all. Without the MPD cryo, i still manage to get
5angstrom, but it has very strong ice-ring signal. I used glycerol (15%,
20%, 25% and 30%) before, but it cracked the crystal.

Please advice.

Thanks

Liew

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Cryo-protectant
From: Liew Chong Wai CWLiew {- at -} NTU {- dot -} EDU {- dot -} SG
Date: 2009-04-23

Next message:
Subject: Re: Cryo-protectant
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-04-24