Re: [ccp4bb] Hanging vs. Sitting

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Call for proposals for biodeuteration [SEC=UNCLASSIFIED]
From: "DUFF, Anthony" adu {- at -} ANSTO {- dot -} GOV {- dot -} AU
Date: 2009-04-30

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Subject: Re: Hanging vs. Sitting
From: Simon Kolstoe s {- dot -} kolstoe {- at -} UCL {- dot -} AC {- dot -} UK
Date: 2009-05-01

Subject: Re: Hanging vs. Sitting
From: Debajyoti Dutta debajyoti_dutta47 {- at -} REDIFFMAIL {- dot -} COM
Date: 2009-05-01

Hi,

From the experiance of mine I can tell you that the crystal size sometimes matters between these two methods. Hanging drop may yield bigger crystals than sitting drop, that may be due to the evaporation rate(surface area). Hanging drop allow us to set different protocols also like free interface diffusion, area covered by the drp etc.

These informations are gained purely by experiance.

cheers

Deb

On Thu, 30 Apr 2009 20:40:35 +0530 wrote

>I have noticed that a significant majority of crystallizations are done in

>hanging- rather than sitting-drop configuration, and considering the

>significant extra labor involved in hanging drops, can only understand this

>preference as a historical bias. I understand that sometimes one technique

>works and not the other, but all things being equal, why is hanging drop

>still "hanging around?" Any insights appreciated...

>

>Jacob Keller

>

>*******************************************

>Jacob Pearson Keller

>Northwestern University

>Medical Scientist Training Program

>Dallos Laboratory

>F. Searle 1-240

>2240 Campus Drive

>Evanston IL 60208

>lab: 847.491.2438

>cel: 773.608.9185

>email: j-keller2@northwestern.edu

>*******************************************

>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Call for proposals for biodeuteration [SEC=UNCLASSIFIED]
From: "DUFF, Anthony" adu {- at -} ANSTO {- dot -} GOV {- dot -} AU
Date: 2009-04-30

Next message:
Subject: Re: Hanging vs. Sitting
From: Simon Kolstoe s {- dot -} kolstoe {- at -} UCL {- dot -} AC {- dot -} UK
Date: 2009-05-01