Re: [ccp4bb] Hanging vs. Sitting

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Hanging vs. Sitting
From: Debajyoti Dutta debajyoti_dutta47 {- at -} REDIFFMAIL {- dot -} COM
Date: 2009-05-01

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Subject: Re: Hanging vs. Sitting
From: Frank von Delft frank {- dot -} vondelft {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-05-01

Subject: Re: Hanging vs. Sitting
From: Simon Kolstoe s {- dot -} kolstoe {- at -} UCL {- dot -} AC {- dot -} UK
Date: 2009-05-01

It's also easier to fish the crystals out of the solution with a
hanging drop.

Simon

On 1 May 2009, at 06:35, Debajyoti Dutta wrote:

>
> Hi,
>
> >From the experiance of mine I can tell you that the crystal size
> sometimes matters between these two methods. Hanging drop may yield
> bigger crystals than sitting drop, that may be due to the
> evaporation rate(surface area). Hanging drop allow us to set
> different protocols also like free interface diffusion, area covered
> by the drp etc.
>
> These informations are gained purely by experiance.
>
> cheers
> Deb
>
>
> On Thu, 30 Apr 2009 20:40:35 +0530 wrote
> >I have noticed that a significant majority of crystallizations are
> done in
> >hanging- rather than sitting-drop configuration, and considering the
> >significant extra labor involved in hanging drops, can only
> understand this
> >preference as a historical bias. I understand that sometimes one
> technique
> >works and not the other, but all things being equal, why is hanging
> drop
> >still "hanging around?" Any insights appreciated...
> >
> >Jacob Keller
> >
> >*******************************************
> >Jacob Pearson Keller
> >Northwestern University
> >Medical Scientist Training Program
> >Dallos Laboratory
> >F. Searle 1-240
> >2240 Campus Drive
> >Evanston IL 60208
> >lab: 847.491.2438
> >cel: 773.608.9185
> >email: j-keller2@northwestern.edu
> >*******************************************
> >
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Hanging vs. Sitting
From: Debajyoti Dutta debajyoti_dutta47 {- at -} REDIFFMAIL {- dot -} COM
Date: 2009-05-01

Next message:
Subject: Re: Hanging vs. Sitting
From: Frank von Delft frank {- dot -} vondelft {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2009-05-01