Re: [ccp4bb] Hanging vs. Sitting

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Cryo-protectant
From: Marcus Winter Marcus {- dot -} Winter {- at -} VARIANINC {- dot -} COM
Date: 2009-05-01

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Date: 2009-05-01

Subject: Re: Hanging vs. Sitting
From: Joe gchen2 {- at -} GMAIL {- dot -} COM
Date: 2009-05-01

Sure, there are differences between these two methods, but no systematic
study has been reported showing one is better than the other in terms of
getting initial hits. Since we have a crystallization robot, I routinely
set up sitting drops for initial screens and hanging drops (manually)
for optimization thereafter. As long as I have no problem getting
reproducible conditions, I will stick to the one I found most efficient
and convenient for myself.

Joe

Frank von Delft wrote:
> Sorry, disagree again: with the right plate type (e.g. SwissCi
> plates), it's far far easier from sitting drop, because:
> 1. you don't have to muck around with flipping over the cover slip,
> instead just cut the seal
> 2. you have more time, because your drop does not evaporate as quickly
> (see earlier mail)
> 3. if the crystal sticks, just poke an acupuncture needle into the
> plastic below it: off it pops.
> phx
>
>
>
> Simon Kolstoe wrote:
>> It's also easier to fish the crystals out of the solution with a
>> hanging drop.
>>
>> Simon
>>
>>
>>
>> On 1 May 2009, at 06:35, Debajyoti Dutta wrote:
>>
>>>
>>> Hi,
>>>
>>> >From the experiance of mine I can tell you that the crystal size
>>> sometimes matters between these two methods. Hanging drop may yield
>>> bigger crystals than sitting drop, that may be due to the
>>> evaporation rate(surface area). Hanging drop allow us to set
>>> different protocols also like free interface diffusion, area covered
>>> by the drp etc.
>>>
>>> These informations are gained purely by experiance.
>>>
>>> cheers
>>> Deb
>>>
>>>
>>> On Thu, 30 Apr 2009 20:40:35 +0530 wrote
>>> >I have noticed that a significant majority of crystallizations are
>>> done in
>>> >hanging- rather than sitting-drop configuration, and considering the
>>> >significant extra labor involved in hanging drops, can only
>>> understand this
>>> >preference as a historical bias. I understand that sometimes one
>>> technique
>>> >works and not the other, but all things being equal, why is hanging
>>> drop
>>> >still "hanging around?" Any insights appreciated...
>>> >
>>> >Jacob Keller
>>> >
>>> >*******************************************
>>> >Jacob Pearson Keller
>>> >Northwestern University
>>> >Medical Scientist Training Program
>>> >Dallos Laboratory
>>> >F. Searle 1-240
>>> >2240 Campus Drive
>>> >Evanston IL 60208
>>> >lab: 847.491.2438
>>> >cel: 773.608.9185
>>> >email: j-keller2@northwestern.edu
>>> >*******************************************
>>> >
>>>
>>>
>>>
>>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Cryo-protectant
From: Marcus Winter Marcus {- dot -} Winter {- at -} VARIANINC {- dot -} COM
Date: 2009-05-01

Next message:
Subject: Computer hardware and OS "survey"
From: "Link,Todd M" tmlink {- at -} MDANDERSON {- dot -} ORG
Date: 2009-05-01