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UV spectrum of the tryptophan containing protein |
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- Protein crystallographyMain steps:- Protein purification- Introduction - Step-by-Step - Common sense - Protocols - Charts & Tables - AM concentration - NaCl-sgar refractometer - Calibration of Superdex 200 - AM table - UV no TRP - UV with TRP - Appendix - Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
Protocols and tips in protein purification or How to purify protein in one day.
UV spectrum of the tryptophan containing proteinThe observed absorbance corresponds to combination of the tryptophan and tyrosine residues in the protein
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