"It's been done (Score:5, Interesting) by SoftwareArtist (1472499) on Friday January 06, 2012 @06:21PM (#38615310) Journal of Failed Crystallization Experiments [proteincry...graphy.org]
Ok, some of the humor is a bit esoteric for those who don't know much molecular biology. You'll just have to take my word for it that it's really funny!
Vitamin D, commonly known as the “sunshine vitamin”, is essential for maintaining calcium metabolism and bone health. The major physiologic function of Vitamin D is to maintain the blood calcium and phosphorus levels within the normal range in order to maintain metabolic functions that are essential for most life processes. Vitamin D3 and Vitamin D2 are metabolized to 25-OH-Vitamin D3 and 25-OH-Vitamin D2 in the liver by the 25-OHase enzyme. The quantitation of these metabolites is widely used for determining of Vitamin D status in humans. The Vitamin D status has clinical significance in a variety of disorders related to hormones. The measurements of the serum concentration of 25-OH-Vitamin D3 and 25-OH-Vitamin D2 have been used for the differential diagnosis of hypocalcemia and hypercalcemic metabolic bone disorders.
Crack (Score:2) I have the most logically organized and beautifully poetic reply to this. It would bring tears of joy to your grandchildren's eyes, but it will not fit in this space. I'll just tweet about it.
The analysis of these metabolites is considered to be difficult. Some reasons for the difficulties are below:
- The low concentrations of 25-OH Vitamin D metabolites in serum (Level of µg/L)
- The low stability in the presence of light and heat
- The presence of other metabolites which have chemical similarity
- The large amount of other related neutral lipids (such as cholesterol)
"irreproducible results" (Score:2) There is a journal entirely devoted to exactly that.
The level of vitamin A in the plasma or serum reflects the quantities of vitamin A and carotene ingested and absorbed by the intestine. Vitamin A plays an essential role in the function of the retina (adaptation to dim light), is necessary for the growth and differentiation of epithelial tissue, and is required for the growth of bone, reproduction, and embryonic development. Together with certain carotenoids, vitamin A enhances immune function, reducing the consequences of some infectious diseases. Vitamin A in excess can be toxic.
Re: And if I remember correctly it was called "The Journal of Irreproducible Results." I probably still have some laying around.
Vitamin E contributes to the normal maintenance of biomembranes, the vascular system, and the nervous system; and provides antioxidant protection for vitamin A. Deficiency of vitamin E in children leads to reversible motor and sensory neuropathies; this problem also has been suspected in adults.
Isn't this what the web is for? (Score:3) by perpenso (1613749) on Friday January 06, 2012 @06:27PM (#38615378) Isn't this what the web is for? Put it on the web, let google index it, and it will be far more accessible than anything else ... "national firewalls" permitting of course.
Deficiencies of vitamins A and E may arise from poor nutrition or from intestinal malabsorption. People, especially children, at risk include those with bowel disease, pancreatic disease, chronic cholestasis, celiac disease, cystic fibrosis, and intestinal lymphangiectasia.
Vitamin A-D3-D2-E Serum LC-MS/MS APCI Analysis Kit was developed for rapid, sensitive, and reliable quantitative detection of the Vitamin A-D3-D2-E-K1 concentrations in human serum samples. It gives results in 10 minutes with a fully automated Zivak MULTITASKER sample preparation and injection system. Main methods and procedures that have been selected are based on EN ISO 13485 and 98/79/EC."
